Essential Oil Against Echinococcus Granulosus, Protoscolicidal Activity of C. Bergamia

Mohammed Jawad Kadhim Al-Anzi; Samim Abbas Hussein; Abdullah Abdulkareem Ahmed Alobaidi; Hayder Hatem Abdulwahhab

doi:10.59890/ijnhs.v3i4.90

Authors

Mohammed Jawad Kadhim Al-Anzi Al-Furat Al-Awsat Technical University
Samim Abbas Hussein University of Babylon
Abdullah Abdulkareem Ahmed Alobaidi University of Baghdad
Hayder Hatem Abdulwahhab Northern Technical University

DOI:

https://doi.org/10.59890/ijnhs.v3i4.90

Keywords:

Citrus Bergamia, Echinococcus Granulosus, Essential Oil, Protoscolicidal Activity, Hydatid Disease

Abstract

Cystic echinococcosis, caused by Echinococcus granulosus, is a zoonotic disease with significant global health and economic impacts. Current treatments, including surgery and chemotherapy, face challenges such as recurrence and toxicity. This study evaluated the in vitro protoscolicidal activity of C. bergamia essential oil against E. granulosus protoscoleces. Hydatid cysts were collected from sheep livers in Karbala, Iraq, and protoscoleces were exposed to four concentrations (1,250, 2,500, 5,000, and 10,000 ppm) of the oil. Viability was assessed using eosin staining at 0 and 10 minutes post-exposure. The oil exhibited concentration- and time-dependent lethality, with 10,000 ppm achieving 100% mortality instantly and lower concentrations reaching complete lethality within 10 minutes. These findings highlight C. bergamia essential oil as a potent natural protoscolicidal agent, offering a promising alternative to conventional therapies. Further in vivo studies are needed to validate its efficacy and safety

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